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中国苹果种质资源丰富,包括大量的野生种、半野生种及栽培品种,在生产中普遍存在同物异名及同名异物现象,不便于种质资源的保存和利用和产业的发展。对取自云南昭通的72份苹果品种(系)进行了遗传多样性分析,基于指纹图谱的基因型构建了分子身份证,并构建了能被快速准确识别的分子二维码,为鉴定苹果种质资源提供了理论依据。
Abstract：China boasts abundant apple germplasm resources,encompassing a large number of wild species,semi-wild species,and cultivated varieties.However,the widespread phenomena of synonymy(different names for the same germplasm) and homonymy(the same name for different germplasms) in production have hindered the conservation and utilization of germplasm resources as well as the development of the apple industry.In this study,genetic diversity analysis was conducted on 72 apple varieties(lines) collected from Zhaotong,Yunnan Province.Molecular identification cards were constructed based on the genotypes derived from fingerprinting,and molecular QR codes enabling rapid and accurate identification were also developed.This research provides a theoretical basis for the identification of apple germplasm resources.
[1]钟艳.云南省昭通市昭阳区永丰镇苹果产业发展浅析[J].河南农业,2016(8):28+30.
[2]李孝敏.昭通市昭阳区苹果产业发展现状及对策[J].现代农业科技,2019(6):71+73.
[3]GIANFRANCESCHI L,SEGLIAS N,TARCHINI R,et al.Simple sequence repeats for the genetic analysis of apple[J].TAGTheoretical and Applied Genetics,1998,96(8):1069-1076.
[4]LIEBHARD R,GIANFRANCESCHI L,KOLLER B,et al.Development and characterisation of 140 new microsatellites in apple[J].Molecular Breeding,2002,10(4):217-241.
[5]尹跃.枸杞品种(系)分子鉴定及亲缘关系分析[D].福州:福建农林大学,2013.
[6]王娟.苹果EST-SSR引物的开发及部分品种亲缘关系分析[D].郑州:河南农业大学,2009.
[7]YEH,FRANCIS C.YEHF.C.et al."PopGene,the userfriendly shareware for population genetic analysis,molecular biology and biotechnology center."(1997).
[8]WACHIRA FN,WAUGH R,HACKETT CA,et al.Detection of genetic diversity in tea(camellia sinensis)using rapd markers.[J].Genome,1995,38(2):201-210.
[9]根井正利.Molecular population genetics and evolution[J].North-Holland Pub.Co.*,1975.
[10]张云秀,杨美玲,于玮玮,等.新疆野苹果SSR-PCR反应体系的优化[J].北方园艺,2017(15):29-35.
[11]高兵,安文杰,孙俊.苹果愈伤组织SSR-PCR反应体系优化[J].北方园艺,2018(10):53-56.
[12]刘玲,陈祥平,范小敏,等.桑树SSR-PCR反应体系的优化[J].江苏农业科学,2019,47(14):45-49+58.
[13]蔡胜文,孙浩元,杨丽,等.仁用杏远缘杂交后代SSR-PCR反应体系的优化[J].中国农学通报,2008(7):50-54.
[14]艾鹏飞,方闪闪,甄志军,等.仁用杏SSR反应体系的建立与优化[J].北方园艺,2009(8):68-70.
[15]高源,刘凤之,王昆,等.基于TP-M13-SSR指纹图谱的中国原产苹果属植物分子身份证的建立[J].植物遗传资源学报,2015,16(6):1290-1297.
[16]丁林静.基于SSR标记的苹果种质资源遗传多样性及亲缘关系研究[D].郑州:河南农业大学,2019.
[17]苏艳丽,毛娟,马宗桓,等.SSR标记的开发在苹果遗传多样性中的应用[J].分子植物育种,2020,18(22):7450-7457.
[18]许茜.苹果的分子身份构建与遗传多样性分析[D].烟台:烟台大学,2021.
[19]李文秀,李进良,贺军军,等.基于SSR标记的砂仁种质资源遗传多样性与群体结构分析[J].中国中药杂志,2022,47(17):4618-4626.
[20]杨阳,刘振,赵洋,等.湖南省主要茶树品种分子指纹图谱的构建[J].茶叶科学,2010,30(5):367-373.
[21]王让剑,杨军,孔祥瑞,等.福建15个茶树品种SSR遗传差异分析与指纹图谱建立[J].福建农业学报,2014,29(10):970-975.
[22]董翔,马梦雪,刘虹,等.基于ISSR图谱构建华中利川和华东苏杭莼菜品种的分子身份证[J].华中农业大学学报,2018,37(4):50-57.
[23]李小梅,赵洪彬,张景涛.芫荽种质资源分子身份证构建[J].中国农学通报,2019,35(31):33-38.
基本信息:
DOI：10.13855/j.cnki.lygs.2025.06.004
中图分类号:S661.1
引用信息:
[1]赵娜斐,刘琳,马钧,等.云南冷凉山地72份苹果种质资源遗传多样性分析及分子身份证的构建[J].落叶果树,2025,57(06):22-27.DOI:10.13855/j.cnki.lygs.2025.06.004.
基金信息:
云南省科技计划重点项目(202401AS070145); 云南省重大科技专项计划(202302AE090005); 云南省院士专家工作站(202505AF350011)
2025-11-10
2025-11-10